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An eDNA/eRNA‐based approach to investigate the life cycle of non‐cultivable shellfish micro‐parasites: the case of Bonamia ostreae , a parasite of the European flat oyster Ostrea edulis ArchiMer
Mérou, Nicolas; Lecadet, Cyrielle; Pouvreau, Stephane; Arzul, Isabelle.
Environmental DNA approaches are increasingly used to detect microorganisms in environmental compartments, including water. They show considerable advantages to study non‐cultivable microorganisms like Bonamia ostreae, a protozoan parasite inducing significant mortality in populations of flat oyster Ostrea edulis . Although B. ostreae development within the host has been well described, questions remain about its behaviour in the environment. As B. ostreae transmission is direct, seawater appears as an interesting target to develop early detection tools and improve our understanding of disease transmission mechanisms. In this context, we have developed an eDNA/eRNA approach allowing detecting and quantifying B. ostreae 18S rDNA/rRNA as well as monitoring...
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Ano: 2020 URL: https://archimer.ifremer.fr/doc/00636/74800/75174.pdf
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Sustainable large‐scale production of European flat oyster ( Ostrea edulis ) seed for ecological restoration and aquaculture: a review ArchiMer
Colsoul, Bérenger; Boudry, Pierre; Pérez‐parallé, María Luz; Bratoš Cetinić, Ana; Hugh‐jones, Tristan; Arzul, Isabelle; Mérou, Nicolas; Wegner, Karl Mathias; Peter, Corina; Merk, Verena; Pogoda, Bernadette.
The conservation and active restoration of European flat oyster (Ostrea edulis) populations are a major focus of ecological restoration efforts to take advantage of the wide‐ranging ecosystem functions and services this species provides. Accordingly, additional and new demands for seed oysters have arisen. In commercial aquaculture (mariculture), the production of O. edulis is still largely based on natural seed collection. Considering the specific requirements, related to ecological restoration, such as the absence of pathogens and the preservation of high genetic diversity, the current supply is insufficient. Despite the development of breeding and controlled reproduction techniques for this species since the late 1930s, seed production today is mainly...
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Ano: 2021 URL: https://archimer.ifremer.fr/doc/00678/79001/81394.pdf
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